SingerLab Online - From The Research Laboratory of Robert H Singer, PhD - Studying the Birth & Travels of RNA
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Request Reagents:

Reagents Available by Request

Please acknowledge the lab if you use these reagents on your publications.

pG14-MS2-GFP:
An episomal yeast expression plasmid containing the MS2-binding protein fused to GFP. This protein also contains a NLS and a HA tag. The auxotropic marker is LEU2.

pSL-MS2-6X, -12X, -24X:
6, 12, or 24 MS2 stem-loops that can be excised with a BamHI/BglII digest. These plasmids must be propagated in Stbl2 competent cells (Invitrogen Cat#10268019)

Mammalian pMS2-GFP:
under the control of the promoter of the large subunit of RNA polymerase II. Contains the SV40 NLS at the C-term of eGFP (see Fusco et al., 2003).

Mammalian pMS2-YFP:
under the control of the promoter of the large subunit of RNA polymerase II. Contains the SV40 NLS at the C-term of eYFP (see Fusco et al., 2003).

eTC GFP ß-actin full length:
under control of ß-actin long promoter. Use with lumio red or lumio green (Invitrogen Cat# 45-7512 or 45-7510 respectively) (see Rodriguez et al., 2006).

eTC GFP ß-actin ΔZip:
under control of ß-actin long promoter. Lacks the ß-actin 3’UTR. Use with lumio red or lumio green (Invitrogen Cat# 45-7512 or 45-7510 respectively) (see Rodriguez et al., 2006).

How to Request Reagents

To request reagents from the Singer Lab, follow the steps below:
 
1.
   Prepare the Simple Letter Material Transfer Agreement (MTA).  
 
a.
   Download and print either the agreement.  
 
b.
   Enter description of material requested. You must use the exact reagent description listed above.  
 
c.
   Enter the name of the provider scientist: "Robert H Singer, PhD".  
 
d.
   Provide all information requested about recipient scientist.  
 
e.
   Get an authorized signature.  
 
f.
   Mail or fax the completed agreement to our university's Office of Biotechnology.
The address and fax number are listed at the top of the agreement.		  
 
 
2.
   Download the SingerLab Request for Reagents Form.  
 
a.
   Mail or fax the completed form to the Singer Lab.
The address and fax number are listed at the top of the form. 		 
 
 
3.
  
The agreement and the form must be sent to the correct destinations! Once the Material Transfer Agreement is approved by our university's Office of Biotechnology our lab will be notified and we will mail the reagent(s) using the information provided in the Request for Reagents Form. We usually need one or two weeks to mail reagents.
  
 
 
4.
  
If you have questions or problems with your request, please contact Robert Singer.
  
 
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