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Singer Lab Publications:

Supplementary Material From Lab Publications
in press.  
A der(8)t(8;11) chromosome in the Karpas-620 myeloma cell line expresses only Cyclin D1: Yet both Cyclin D1 and MYC are repositioned in close proximity to the 3′ IGH enhancer.
Dib A, Glebov OK, Shou Y, Singer RH, Kuehl WM
DNA Repair in press (2009)
Abstract | Link to Journal | Download Reprint (PDF)
 

209.

  
Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells.
Maier S, Daroqui MC, Scherer S, Roepcke S, Velcich A, Shenoy SM, Singer RH, Augenlicht LH
J Cell Physiol 218(3):638-642 (2009 March)
Abstract | Link to Journal | Download Reprint (PDF)
 
208.  
Cell Biology of mRNA Decay.
Grünwald D, Singer RH, Czaplinski K
Methods Enzymol 448(27):553-577 (2008 December 15)
Abstract | Link to Book Series | Download Reprint (PDF) | ISBN: 978-0123743787
 
207.  
RNA in Cells.
de Turris V, Singer RH
Single Molecule Dynamics in Life Science, eds Yanagida T and Ishii Yoshiharu, 171-190. Wiley-VCH (2008 December 15)
ISBN: 978-3527312887
 
206.  
Single-RNA counting reveals alternative modes of gene expression in yeast.
Zenklusen D, Larson DR, Singer RH
Nat Struct Mol Biol 15(12):1263-1271 (2008 December)
Abstract | Link to Journal | Download Reprint (PDF) | Download Supplemental Material (PDF)
 
205.  
Calibrating excitation light fluxes for quantitative light microscopy in cell biology.
Grünwald D, Shenoy SM, Burke S, Singer RH
Nat Protoc 3(11):1809-1814 (2008 November)
Abstract | Link to Journal | Download Reprint (PDF)
 
204.  
The Dynamics of Mammalian P Body Transport, Assembly and Disassembly In Vivo.
Aizer A, Brody Y, Ler LW, Sonenberg N, Singer RH, Shav-Tal Y
Mol Biol Cell 19(10):4154-4166 (2008 October)
Abstract | Link to Journal | Download Reprint (PDF) | Supplemental Online Information
 
203.  
Highways for mRNA Transport
Singer RH
Cell 134(5):722-723 (2008 September 5)
Abstract | Link to Journal | Download Reprint (PDF)
 
202.  
Feedback regulation between ZBP1 and β-catenin mRNAs in breast cancer cells.
Gu W, Wells AL, Pan F, Singer RH
Mol Cell Biol 28(16):4963-4974 (2008 August)
Abstract | Link to Journal | Download Reprint (PDF)
 
201.  
Nuclear microenvironment in cancer diagnosis and treatment.
Pezo RC, Singer RH
J Cell Biochem 104(6):1953-1963 (2008 August 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
200.  
A role for VICKZ proteins in the progression of colorectal carcinomas: Regulating lamellipodia formation.
Vainer G, Vainer-Mossel E, Pikarsky A, Shenoy SM, Oberman F, Yeffet A, Singer RH, Pikarsky E, Yisraeli JK
J Pathol 215(4):445-456 (2008 August)
Abstract | Link to Journal | Download Reprint (PDF)
 
199.  
Single-Cell Transcription Site Activation Predicts Chemotherapy Response in Human Colorectal Tumors.
Pezo RC, Gandhi SJ, Shirley LA, Pestell RG, Augenlicht LH, Singer RH
Cancer Res 68(13):4977-4982 (2008 July 1)
Abstract | Link to Journal | Download Reprint (PDF)
 
198.  
A Direct Role for FMRP in Activity-Dependent Dendritic mRNA Transport Links Filopodial-Spine Morphogenesis to Fragile X Syndrome.
Dictenberg JB, Swanger SA, Antar LN, Singer RH, Bassell GJ
Dev Cell 14(6):926-939 (2008 June 10)
Abstract | Link to Journal | Download Reprint (PDF)
 
197.  
The Dynamic Range of Transcription.
Darzacq X, Singer RH
Mol Cell 30(5):545-546 (2008 June 5)
Abstract | Link to Journal | Download Reprint (PDF)
 
196.  
Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation.
Deng Y, Singer RH, Gu W
Genes Dev 22(8):1037-1050 (2008 April 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
195.  
Mechanisms and cellular roles of local protein synthesis in mammalian cells.
Rodriguez AJ, Czaplinski K, Condeelis JS, Singer RH
Curr Opin Cell Biol 20(2):144-149 (2008 April)
Abstract | Link to Journal | Download Reprint (PDF)
 
194.  
Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid–protein complex that contains mRNAs and subunits of the RNA-processing body.
Chang W, Zaarour RF, Reck-Peterson S, Rinn J, Singer RH, Snyder M, Novick P, Mooseker MS
RNA 14(3):491-502 (2008 March)
Abstract | Link to Journal | Download Reprint (PDF)
 
193.  
Structural basis for the coevolution of a viral RNA–protein complex.
Chao JA, Patskovsky Y, Almo SC, Singer RH
Nat Struct Mol Biol 15(1):103-105 (2008 January)
Abstract | Link to Journal | Download Reprint (PDF) | Download Reprint of Research Highlights (PDF)
 
192.  
Imaging Real-Time Gene Expression in Living Systems.
Wells AL, Condeelis JS, Singer RH, Zenklusen D
Single-Molecule Techniques: A Laboratory Manual, eds Selvin PR and Ha T, 209-238. Cold Spring Harbor Laboratory Press (2007 December)
ISBN: 978-087969775-4
 
191.  
ZBP2 facilitates binding of ZBP1 to β-actin mRNA during transcription.
Pan F, Huttelmaier S, Singer RH, Gu W
Mol Cell Biol 27(23):8340-8351 (2007 December)
Abstract | Link to Journal | Download Reprint (PDF)
 
190.  
The spatial order of transcription in mammalian cells.

Levsky JM, Shenoy SM, Chubb JR, Hall CB, Capodieci P, Singer RH
J Cell Biochem 102(3):609-617 (2007 October 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
189.  
ZBP1 enhances cell polarity and reduces chemotaxis.

Lapidus K, Wyckoff J, Mouneimne G, Lorenz M, Soon L, Condeelis JS, Singer RH
J Cell Science 120(18):3173-3178 (2007 September 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
188.  
In vivo dynamics of RNA polymerase II transcription.
Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, Singer RH
Nat Struct Mol Biol 14(9):796-806 (2007 September)
Abstract | Link to Journal | Download Reprint (PDF) | Download Reprint of News & Views (PDF)
 
187.  
QNQKE targeting motif for the SMN-Gemin multiprotein complexin neurons.
Zhang HL, Xing L, Singer RH, Bassell GJ
J Neurosci Res 85(12):2657-2667 (2007 September)
Abstract | Link to Journal | Download Reprint (PDF)
 
186.  
Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT).
Loudig O, Milova E, Brandwein-Gensler M, Massimi A, Belbin TJ, Childs G, Singer RH, Rohan T, Prystowsky MB
Nucleic Acids Res 35(15):e94 (2007 August)
Abstract | Link to Journal | Download Reprint (PDF)
 
185.  
Yeast Mitochondrial Transcriptomics.
Garcia M, Darzacq X, Devaux F, Singer RH, Jacq C
Methods in Molecular Biology: Mitochondria Practical Protocols, eds Leister D and Herrmann J. Humana Press (2007 June 12)
ISBN: 978-1-58829-667-2
 
184.  
Dissecting Cellular Activity from Single Genes to Single mRNAs.
Darzacq X, Singer RH, Shav-Tal Y
New Frontiers in Ultrasensitive Bioanalysis: Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Cell Analysis, ed Xu XN, 29-39. Wiley-Interscience (2007 April 13)
Abstract | Download Reprint (PDF) | ISBN: 978-0-471-74660-7
 
183.  
Imaging mRNA movement from transcription sites to translation sites.
Rodriguez AJ, Condeelis J, Singer RH, Dictenberg JB
Semin Cell Dev Biol 18(2):202-208 (2007 April)
Abstract | Link to Journal | Download Reprint (PDF)
 
182.  
Localized Translation through Messenger RNA Localization.
Gavis ER, Singer RH, Huttelmaier S
Translational Control in Biology and Medicine, eds Mathews MB and Hershey J, 689-718. Cold Spring Harbor Laboratory Press (2007 March)
ISBN: 0-87-969767-9
 
181.  
YRA1 Autoregulation Requires Nuclear Export and Cytoplasmic Edc3p-Mediated Degradation of Its Pre-mRNA.
Dong S, Li C, Zenklusen D, Singer RH, Jacobson A, He F
Mol Cell 25(4):559-573 (2007 February 23)
Abstract | Link to Journal | Download Reprint (PDF)
 
180.  
Mitochondria-associated Yeast mRNAs and the Biogenesis of Molecular Complexes.
Garcia M, Darzacq X, Delaveau T, Jourdren L, Singer RH, Jacq C
Mol Biol Cell 18(2):362-368 (2007 February)
Abstract | Link to Journal | Download Reprint (PDF)
 
179.  
Dynamic association and localization of human H/ACA RNP proteins.
Kittur N, Darzacq X, Roy S, Singer RH, Meier UT
RNA 12(12):2057-2062 (2006 December)
Abstract | Link to Journal | Download Reprint (PDF)
 
178.  
Pathways for mRNA localization in the cytoplasm.
Czaplinski K, Singer RH
Trends Biochem Sci 31(12):687-693 (2006 December)
Abstract | Link to Journal | Download Reprint (PDF)
 
177.  
ZBP1 regulates mRNA stability during cellular stress.
Stöhr N, Lederer M, Reinke C, Meyer S, Hatzfeld M, Singer RH, Huttelmaier S
J Cell Biol 175(4):527-534 (2006 November 20)
Abstract | Link to Journal | Download Reprint (PDF)
 
176.  
Visualization of mRNA translation in living cells.
Rodriguez AJ, Shenoy SM, Singer RH, Condeelis J
J Cell Biol 175(1):67-76 (2006 October 9)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
175.  
A peptide motif in Raver1 mediates splicing repression by interaction with the PTB RRM2 domain.
Rideau AP, Gooding C, Simpson PJ, Monie TP, Lorenz M, Huttelmaier S, Singer RH, Matthews S, Curry S, Smith CW
Nat Struct Mol Biol 13(9):839-848 (2006 September)
Abstract | Link to Journal | Download Reprint (PDF) | Download Supplement (PDF)
 
174.  
Multiprotein Complexes of the Survival of Motor Neuron Protein SMN with Gemins Traffic to Neuronal Processes and Growth Cones of Motor Neurons.
Zhang HL, Xing L, Rossoll W, Wichterle H, Singer RH, Bassell GJ
J Neurosci 26(33):8622-8632 (2006 August 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
173.  
Gene expression within a dynamic nuclear landscape.
Shav-Tal Y, Darzacq X, Singer RH
EMBO J 25(15):3469-3479 (2006 August)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
172.  
Nucleus and gene regulation.
Pederson T, Singer RH
Curr Opin Cell Biol 18(3):229-230 (2006 June)
Abstract | Link to Journal | Download Reprint (PDF)
 
171.  
Transcriptional pulsing of a developmental gene.
Chubb JR, Trcek T, Shenoy SM, Singer RH
Curr Biol 16(10):1018-1025 (2006 May 23)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
170.  
Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells.
Darzacq X, Kittur N, Roy S, Shav-Tal Y, Singer RH, Meier UT
J Cell Biol 173(2):207-219 (2006 April 24)
Abstract | Link to Journal | Download Reprint (PDF)
 
169.  
Developmental timing in Dictyostelium is regulated by the Set1 histone methyltransferase.
Chubb JR, Bloomfield G, Xu Q, Kaller M, Ivens A, Skelton J, Turner BM, Nellen W, Shaulsky G, Kay RR, Bickmore WA, Singer RH
Dev Biol 292(2):519-532 (2006 April 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
168.  
Assembling an intermediate filament network by dynamic cotranslation.
Chang L, Shav-Tal Y, Trcek T, Singer RH, Goldman RD
J Cell Biol 172(5):747-758 (2006 February 27)
Abstract | Link to Journal | Download Reprint (PDF)
 
167.  
Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1.
Huttelmaier S, Zenklusen D, Lederer M, Dictenberg J, Lorenz M, Meng X, Bassell GJ, Condeelis J, Singer RH
Nature 438(7067):512-515 (2005 November 24)
Abstract | Link to Journal | Download Reprint (PDF) | Download Supplement (PDF) |
Download Reprint of News & Views by Dahm & Kiebler (PDF) | Download Reprint of News & Views by Paquin & Chartrand (PDF)
 
166.  
Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH): Application to the Analysis of Cultured Cells.
Levsky JM, Braut SA, Singer RH
Cell Biology: A Laboratory Handbook Volume 4, eds Celis JE, et al, 121-130. Academic Press (2005 November 16)
ISBN: 0-12-164730-7
 
165.  
The Great Escape: When Cancer Cells Hijack the Genes for Chemotaxis and Motility.
Condeelis J, Singer RH, Segall JE
Annu Rev Cell Dev Biol 21:695-718 (2005 November)
Abstract | Link to Publisher | Download Reprint (PDF)
 
164.  
In Situ Hybridization to RNA.
Huang S, Singer RH
Basic Methods in Microscopy, eds Spector DL and Goldman RD, 277-290. Cold Spring Harbor Laboratory (2005 October 28)
ISBN: 0-87969-751-2, 0-87969-747-4
 
163.  
Imaging of gene expression in living cells and tissues.
Singer RH, Lawrence DS, Ovryn B, Condeelis J
J Biomed Optics 10(5):0514061-0514069 (2005 September/October)
Abstract | Link to Publisher | Download Reprint (PDF)
 
162.  
RNA localization.
Shav-Tal Y, Singer RH
J Cell Sci 118(18):4077-4081 (2005 September 15)
Abstract | Link to Journal | Poster Insert | Download Reprint (PDF)
 
161.  
Gene expression profiling in single cells within tissue.
Capodieci P, Donovan M, Buchinsky H, Jeffers Y, Cordon-Cardo C, Gerald W, Edelson J, Shenoy SM, Singer RH
Nat Methods 2(9):663-665 (2005 September)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
160.  
Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts.
Mingle LA, Okuhama NN, Shi J, Singer RH, Condeelis J, Liu G
J Cell Sci 118(11):2425-2433 (2005 June 1)
Abstract | Link to Journal | Download Reprint (PDF)
 
159.  
Dynamics of transcription and mRNA export.
Darzacq X, Singer RH, Shav-Tal Y
Curr Opin Cell Biol 17(3):332-339 (2005 June)
Abstract | Link to Journal | Download Reprint (PDF)
 
158.  
Promotion of importin alpha-mediated nuclear import by the phosphorylation-dependent binding of cargo protein to 14-3-3.
Faul C, Huttelmaier S, Oh J, Hachet V, Singer RH, Mundel P
J Cell Biol 169(3):415-424 (2005 May 9)
Abstract | Link to Journal | Download Reprint (PDF)
 
157.  
Dynamic Sorting of Nuclear Components into Distinct Nucleolar Caps during Transcriptional Inhibition.
Shav-Tal Y, Blechman J, Darzacq X, Montagna C, Dye BT, Patton JG, Singer RH, Zipori D
Mol Biol Cell 16(5):2395-2413 (2005 May)
Abstract | Link to Journal | Download Reprint (PDF)
 
156.  
How and why does beta-actin mRNA target?
Condeelis JS, Singer RH
Biol Cell 97(1):97-110 (2005 January)
Abstract | Link to Journal | Download Reprint (PDF)
 
155.  
Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors.
Wang W, Goswami S, Lapidus K, Wells AL, Wyckoff JB, Sahai E, Singer RH, Segall JE, Condeelis JS
Cancer Res 64(23):8585-8594 (2004 December 1)
Abstract | Link to Journal | Download Reprint (PDF)
 
154.  
She2p Is a Novel RNA Binding Protein with a Basic Helical Hairpin Motif.
Niessing D, Huttelmaier S, Zenklusen D, Singer RH, Burley SK
Cell 119(4):491-502 (2004 November 12)
Abstract | Link to Journal | Download Reprint (PDF)
 
153.  
Imaging of Single mRNAs in the Cytoplasm of Living Cells.
Shav-Tal Y, Shenoy SM, Singer RH
Live Cell Imaging: A Laboratory Manual, eds Goldman RD and Spector DL, 603-615. Cold Spring Harbor Laboratory (2004 October)
ISBN: 0-87969-682-6, 0-87969-683-4
 
152.  
Imaging gene expression in single living cells.
Shav-Tal Y, Singer RH, Darzacq X
Nat Rev Mol Cell Biol 5(10):855-862 (2004 October)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
151.  
Dynamics of Single mRNPs in Nuclei of Living Cells.
Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL, Singer RH
Science 304(5678):1797-1800 (2004 June 18)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
150.  
A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization.
Gu W, Deng Y, Zenklusen D, Singer RH
Genes Dev 18(12):1452-1465 (15 June 2004)
Abstract | Link to Journal | Download Reprint (PDF)
 
149.  
Imaging sites of N-WASP activity in lamellipodia and invadopodia of carcinoma cells.
Lorenz M, Yamaguchi H, Wang Y, Singer RH, Condeelis J
Curr Biol 14(8):697-703 (20 April 2004)
Abstract | Link to Journal | Download Reprint (PDF) | Download Supplement (PDF)
 
148.  
From Silencing to Gene Expression: Real-Time Analysis in Single Cells.
Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL
Cell 116(5):683-698 (2004 March 5)
Abstract | Link to Journal | Download Reprint (PDF)
 
147.  
Measurement of barbed ends, actin polymerization, and motility in live carcinoma cells after growth factor stimulation.
Lorenz M, DesMarais V, Macaluso F, Singer RH, Condeelis J
Cell Motil Cytoskeleton 57(4):207-217 (2004 April)
Abstract | Link to Journal | Download Reprint (PDF)
 
146.  
Imaging of Single mRNAs in the Cytoplasm of Living Cells.
Fusco D, Bertrand E, Singer RH
RNA Trafficking and Nuclear Structure Dynamics, ed Jeanteur P, 135-150. Springer-Verlag (2004 February 29)
Prog Mol Subcell Biol 35:135-150. (2004)
Download Reprint (PDF) | ISBN: 3-540-40451-1
 
145.  
The PTB interacting protein raver1 regulates alpha-tropomyosin alternative splicing.
Gromak N, Rideau A, Southby J, Scadden AD, Gooding C, Huttelmaier S, Singer RH, Smith CW
EMBO J 22(23):6356-6364 (2003 December 1)
Abstract | Link to Journal | Download Reprint (PDF)
 
144.  
RNA asymmetric distribution and daughter/mother differentiation in yeast.
Darzacq X, Powrie E, Gu W, Singer RH, Zenklusen D
Curr Opin Microbiol 6(6):1-7 (2003 December)
Abstract | Link to Journal | Download Reprint (PDF)
 
143.  
Localization of a ß-Actin Messenger Ribonucleoprotein Complex with Zipcode-Binding Protein Modulates the Density of Dendritic Filopodia and Filopodial Synapses.
Eom T, Antar LN, Singer RH, Bassell GJ
J Neurosci 23(32):10433-10444 (2003 November 12)
Abstract | Link to Journal | Download Reprint (PDF)
 
142.  
RNA Localization and Signal Transduction.
Latham V, Singer RH
Handbook of Cell Signalling Volume 3, ed Bradshaw RA and Dennis EA, 293-297. Academic Press (2003 November 7)
Download Reprint (PDF) | ISBN: 0-12-124546-2
 
141.  
   
RNA localization: visualization in real-time.
Singer RH
Curr Biol 13(17):R673-R675 (2003 September 2)
Abstract | Link to Journal | Download Reprint (PDF)
 
140.  
Role of the parafusin orthologue, PRP1, in microneme exocytosis and cell invasion in Toxoplasma gondii.
Matthiesen SH, Shenoy SM, Kim K, Singer RH, Satir BH
Cell Microbiol 5(9):613-624 (2003 September)
Abstract | Link to Journal | Download Reprint (PDF)
 
139.  
Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization.
Zhang HL, Pan F, Hong D, Shenoy SM, Singer RH, Bassell GJ
J Neurosci 23(16):6627-6637 (2003 July 23)
Abstract | Link to Journal | Supplemental Online Information | Download Reprint (PDF)
 
138.  
Fluorescence in situ hybridization: past, present and future.
Levsky JM, Singer RH
J Cell Sci 116(14):2833-2838 (2003 July 15)
Abstract | Link to Journal | Download Reprint (PDF)
 
137.  
Asymmetric Distribution of Nuclear Pore Complexes and the Cytoplasmic Localization of ß2-Tubulin mRNA in Chlamydomonas reinhardtii.
Colón-Ramos DA, Salisbury JL, Sanders MA, Shenoy SM, Singer RH, Garciá-Blanco MA
Dev Cell 4(6):941-952 (2003 June)
Abstract | Link to Journal | Download Reprint (PDF)
 
136.  
Activity-Dependent Trafficking and Dynamic Localization of Zipcode Binding Protein 1 and beta-Actin mRNA in Dendrites and Spines of Hippocampal Neurons.
Tiruchinapalli DM, Oleynikov Y, Kelic S, Shenoy SM, Hartley A, Stanton PK, Singer RH, Bassell GJ