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| < previous patent | all patents | next patent > |
| #5,985,549 |
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Improved
methodologies for in-situ hybridization and non-isotopic detection of
nucleic acid sequences are provided which offer major increases of resolution,
sensitivity, and simplicity unavailable in previously known techniques.
The methodology is able to detect even a single-copy of a specific nucleic
acid of interest under controlled conditions regardless of whether these
are DNA or RNA sequences; or whether the nucleic acid sequence of interest
is localized in the chromosomes, nucleus, or cytoplasm of a cell. The
methods employ a variety of non-isotopic labels and detection means for
rapid and reliable assays. The invention is also provided in kit form
for use in the clinical/diagnostic laboratory such that a relatively unskilled
person can accurately and reproducibly detect even a single-copy of a
specific nucleic acid of interest. |
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